The Mona Lisa's portrayal of individuality has had a significant impact on the concept of identity in art by showcasing a sense of mystery and enigma that has captivated viewers for centuries. The painting's subtle smile and direct gaze have inspired artists to explore the complexities of human identity and emotion in their own work. Additionally, the Mona Lisa's unique depiction of a specific individual has challenged traditional notions of portraiture and encouraged artists to experiment with new ways of representing personal identity in art.