The sibling-like relationship between Yamaguchi and Tsukishima in the anime "Haikyuu!!" is significant because it showcases a deep bond of friendship and support. Despite their differences in personalities, they complement each other and provide emotional support, encouragement, and motivation to each other both on and off the volleyball court. Their relationship also highlights the theme of growth and loyalty, as they challenge each other to become better players and individuals. Additionally, their dynamic adds depth and complexity to the story, enriching the overall narrative of friendship and teamwork in the series.