Multi-factor authentication plays a crucial role in enhancing security for Bitcoin wallet access by adding an extra layer of protection beyond just a password. This additional layer typically requires the user to provide two or more forms of verification before accessing the wallet, such as a password combined with a unique code sent to their mobile device or generated by an authentication app. By requiring multiple factors to authenticate, multi-factor authentication significantly reduces the risk of unauthorized access to the Bitcoin wallet, making it more secure against various types of cyber threats.